7N5V

ZBTB7A Zinc Finger Domain Bound to DNA Duplex Containing GGACCC (Oligo 20)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-03-06
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00000
Spacegroup name	P 1 21 1
Unit cell lengths	51.292, 60.067, 133.729
Unit cell angles	90.00, 98.50, 90.00

Refinement procedure

Resolution	43.470 - 3.080
R-factor	0.2627
Rwork	0.260
R-free	0.31360
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7n5u
RMSD bond length	0.004
RMSD bond angle	0.576
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.470	3.200
High resolution limit [Å]	3.080	3.080
Rmerge	0.142	0.531
Rpim	0.067	0.274
Number of reflections	27875	2492
<I/σ(I)>	7.8	1.8
Completeness [%]	96.0	86.7
Redundancy	5.4	4.3
CC(1/2)		0.992

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	289	0.1 M Bis-Tris, pH 6.5, 20% PEG5000 MME