7N5N

PCNA from Thermococcus gammatolerans: crystal III, collection 15, 2.20 A, 28.7 MGy

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-04-25
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	P 3
Unit cell lengths	93.056, 93.056, 63.976
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.095 - 2.200
R-factor	0.1788
Rwork	0.176
R-free	0.22570
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5a6d
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.095	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.131	0.986
Number of reflections	31432	3150
<I/σ(I)>	19.02	2.37
Completeness [%]	99.6	99.75
Redundancy	5.1	5.1
CC(1/2)	0.999	0.748

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	5.2	291	100 mM sodium citrate pH 5.2, 3.0 M ammonium sulfate, 7.5% MPD