7N5J
PCNA from Thermococcus gammatolerans: crystal I, collection 5, 2.82 A, 89.1 MGy
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-04-25 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9795 |
Spacegroup name | P 63 |
Unit cell lengths | 187.870, 187.870, 64.314 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 37.326 - 2.820 |
R-factor | 0.1736 |
Rwork | 0.170 |
R-free | 0.23300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5a6d |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.330 | 2.920 |
High resolution limit [Å] | 2.820 | 2.820 |
Rmerge | 0.152 | 1.205 |
Number of reflections | 31591 | 3147 |
<I/σ(I)> | 18.68 | 2.43 |
Completeness [%] | 99.8 | 99.9 |
Redundancy | 10.3 | 10.5 |
CC(1/2) | 0.979 | 0.797 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 5.2 | 291 | 100 mM sodium citrate pH 5.2, 3.2 M ammonium sulfate, 7.5% MPD |