7N57

Crystal structure of R20A human Galectin-7 mutant in presence of lactose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-09
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97952
Spacegroup name	P 21 21 21
Unit cell lengths	30.220, 76.190, 111.190
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.910 - 1.830
R-factor	0.173
Rwork	0.171
R-free	0.20820
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4gal
RMSD bond length	0.011
RMSD bond angle	1.206
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (2.7.12)
Refinement software	PHENIX (1.19.2-4158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	44.910	44.910	1.500
High resolution limit [Å]	1.460	6.530	1.460
Rmerge	0.186	0.119	1.755
Rmeas	0.202	0.133	1.890
Total number of observations	322349
Number of reflections	45632	599	3336
<I/σ(I)>	5.12	10.8	1.01
Completeness [%]	100.0	99.7	100
Redundancy	7.064	5.953	7.204
CC(1/2)	0.986	0.985	0.346

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	0.2M Sodium chloride, 0.1M Tris pH 8.0, 20% w/v PEG 6000