7N50

Structure of a bacterial gasdermin from Bradyrhizobium tropiciagri

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	80
Detector technology	PIXEL
Collection date	2020-11-04
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97918
Spacegroup name	P 21 21 2
Unit cell lengths	40.770, 146.180, 34.980
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.270 - 1.500
R-factor	0.1909
Rwork	0.188
R-free	0.22020
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.052
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX (1.19.1_4122)
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.770	1.530
High resolution limit [Å]	1.500	1.500
Rpim	0.038	0.787
Number of reflections	33993	1639
<I/σ(I)>	12.3	2
Completeness [%]	98.7
Redundancy	7.4
CC(1/2)	0.998	0.216

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.4	291	100 mM sodium acetate (pH 4.4) and 23% PEG-3350