7N4P

Crystal Structure of Lizard Cadherin-23 EC1-2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2016-12-18
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97920
Spacegroup name	P 41 21 2
Unit cell lengths	43.386, 43.386, 296.227
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.671 - 2.097
Rwork	0.211
R-free	0.26650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2whv
RMSD bond length	0.014
RMSD bond angle	1.857
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.140
High resolution limit [Å]	2.097	2.097
Rmeas	0.106	0.589
Number of reflections	17931	736
<I/σ(I)>	16.65
Completeness [%]	98.3	86
Redundancy	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	277	0.1 M MES pH 6.5 1.1 M CaCl2