7N43
Alpha-conotoxin OmIA with unusual pharmacological properties at alpha7 nicotinic receptors
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-12-13 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 169.363, 169.363, 124.343 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.190 - 2.470 |
| R-factor | 0.18748 |
| Rwork | 0.187 |
| R-free | 0.21242 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5t90 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.685 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.240 | 2.530 |
| High resolution limit [Å] | 2.470 | 2.470 |
| Number of reflections | 65087 | 4285 |
| <I/σ(I)> | 17.9 | |
| Completeness [%] | 99.6 | |
| Redundancy | 26.85 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2 M ammonium sulfate, 5% PEG4000 and 0.1M sodium acetate trihydrate pH 4.6 |
