7N40

Crystal structure of LIN9-RbAp48-LIN37, a MuvB subcomplex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-26
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.033
Spacegroup name	C 1 2 1
Unit cell lengths	133.580, 77.810, 64.560
Unit cell angles	90.00, 114.71, 90.00

Refinement procedure

Resolution	39.489 - 2.550
R-factor	0.1681
Rwork	0.159
R-free	0.24880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3gfc
RMSD bond length	0.007
RMSD bond angle	0.921
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.15.2_3472: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.000	2.690
High resolution limit [Å]	2.550	2.550
Rmerge	0.135	0.520
Number of reflections	19131	2805
<I/σ(I)>	7.9	3.5
Completeness [%]	97.4	97.8
Redundancy	2.3	2.3
CC(1/2)	0.970	0.470

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	0.1 M Bis-Tris, pH 6.5, 20% PEG3350, 0.2 M sodium tartrate