7N2Q

AS4.3-YEIH-HLA*B27

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-1
Synchrotron site	SSRL
Beamline	BL12-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-12-19
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97946
Spacegroup name	P 1 21 1
Unit cell lengths	54.977, 97.412, 175.484
Unit cell angles	90.00, 92.33, 90.00

Refinement procedure

Resolution	47.420 - 2.700
R-factor	0.2155
Rwork	0.213
R-free	0.26400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5KSB 1JGE
RMSD bond length	0.002
RMSD bond angle	0.524
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.19.1_4122+SVN)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.710	2.790
High resolution limit [Å]	2.700	2.700
Number of reflections	49539	4552
<I/σ(I)>	2.7
Completeness [%]	97.4
Redundancy	3.4
CC(1/2)	0.938	0.346

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	25% PEG3350 with 0.2M Ammonium sulfate and 0.1M BIS-TRIS pH 6.5