7N2B
A DARPin semi-rigidly fused to the 3TEL crystallization chaperone
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-10-21 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979460 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.962, 63.625, 166.005 |
| Unit cell angles | 90.00, 90.16, 90.00 |
Refinement procedure
| Resolution | 40.160 - 3.221 |
| R-factor | 0.2273 |
| Rwork | 0.226 |
| R-free | 0.24250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qar 4j7w |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.563 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.160 | 3.336 |
| High resolution limit [Å] | 3.221 | 3.221 |
| Rmerge | 0.071 | 0.506 |
| Rmeas | 0.084 | 0.605 |
| Rpim | 0.328 | |
| Number of reflections | 13842 | 1491 |
| <I/σ(I)> | 10.98 | 2.46 |
| Completeness [%] | 87.3 | 95.36 |
| Redundancy | 3.3 | 3.3 |
| CC(1/2) | 0.999 | 0.906 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 298 | 200 mM L-Proline, 100 mM HEPES, 10 % w/v Polyethylene glycol 3350 |
