7N27
Crystal Structure of chromodomain of CDYL in complex with inhibitor UNC6261
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-07-29 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97934 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 62.971, 76.386, 80.628 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.640 - 1.850 |
| R-factor | 0.227 |
| Rwork | 0.225 |
| R-free | 0.26200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6v41 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.572 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.870 |
| High resolution limit [Å] | 1.840 | 1.840 |
| Rmerge | 0.083 | 0.752 |
| Number of reflections | 33365 | |
| <I/σ(I)> | 10.5 | |
| Completeness [%] | 99.3 | 98.8 |
| Redundancy | 5.9 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | 3.5M Na Formate, 0.1M Bis-Tris propane pH7.0 |
