7N1J

Crystal structure of FGFR4 domain 3 in complex with a de novo-designed mini-binder

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-03-10
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 63
Unit cell lengths	107.531, 107.531, 69.047
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.560 - 2.990
R-factor	0.2099
Rwork	0.209
R-free	0.23290
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1cvs
RMSD bond length	0.003
RMSD bond angle	0.481
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.170
High resolution limit [Å]	2.990	2.990
Number of reflections	9194	1452
<I/σ(I)>	19.1
Completeness [%]	98.7
Redundancy	4.7
CC(1/2)	0.998	0.952

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277.15	0.2 M sodium chloride, 0.1 M MES (pH 6.0), 20% PEG 3,350