7MZY

Anaplastic lymphoma kinase (ALK) extracellular fragment of ligand binding region 673-986

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-21
Detector	DECTRIS PILATUS 2M
Spacegroup name	P 21 21 2
Unit cell lengths	70.289, 133.356, 62.909
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.570 - 1.500
R-factor	0.1698
Rwork	0.168
R-free	0.19650
Structure solution method	MAD
RMSD bond length	0.012
RMSD bond angle	1.154
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.530
High resolution limit [Å]	1.500	1.500
Number of reflections	95109	2895
<I/σ(I)>	22.4
Completeness [%]	92.3	61.6
Redundancy	11.6	7.6
CC(1/2)	0.985	0.874

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	293.15	25.5 % PEG 4000, 15% glycerol, 0.17 M ammonium acetate and 0.085M sodium citrate pH 5.6
1	VAPOR DIFFUSION, HANGING DROP	5.6	293.15	25.5 % PEG 4000, 15% glycerol, 0.17 M ammonium acetate and 0.085M sodium citrate pH 5.6