7MZ0
Structure of human DNA polymerase beta complexed with dzA as the template base in a 1-nucleotide gapped DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.3 |
| Synchrotron site | ALS |
| Beamline | 5.0.3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-10-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97648 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.649, 79.172, 54.819 |
| Unit cell angles | 90.00, 106.27, 90.00 |
Refinement procedure
| Resolution | 19.793 - 2.021 |
| R-factor | 0.2239 |
| Rwork | 0.221 |
| R-free | 0.27570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bpx |
| RMSD bond length | 0.003 |
| RMSD bond angle | 1.073 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 20.000 | 20.000 | 2.050 |
| High resolution limit [Å] | 2.020 | 5.450 | 2.020 |
| Rmerge | 0.079 | 0.034 | 0.469 |
| Total number of observations | 124548 | ||
| Number of reflections | 29323 | 1507 | 1429 |
| <I/σ(I)> | 8 | ||
| Completeness [%] | 99.8 | 99.7 | 98.1 |
| Redundancy | 4.2 | 4.3 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 14% to 23% PEG3400, and 350 mM sodium acetate in 50 mM imidazole (pH 7.5) |
