7MYH

Ubiquitin variant UbV.k.2 in complex with Ube2k

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-25
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	0.9537
Spacegroup name	P 21 21 21
Unit cell lengths	38.841, 76.088, 135.482
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.835 - 2.394
R-factor	0.2084
Rwork	0.206
R-free	0.24870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5dfl
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.835	2.480
High resolution limit [Å]	2.390	2.390
Rmerge	0.090	1.401
Rmeas	0.099	1.540
Number of reflections	16440	9474
<I/σ(I)>	12.8
Completeness [%]	99.1	97.6
Redundancy	5.5	5.8
CC(1/2)	0.999	0.551

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	289	0.2 M sodium citrate tribasic trihydrate, 0.1 M Bis-Tris propane, pH 7.5, 20% PEG3350