7MYG
M. tb Ag85C modified by THL-10d
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2021-02-16 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9787 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.458, 70.606, 68.590 |
| Unit cell angles | 90.00, 89.97, 90.00 |
Refinement procedure
| Resolution | 59.460 - 2.510 |
| R-factor | 0.1623 |
| Rwork | 0.155 |
| R-free | 0.22240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dqz |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.460 | 2.600 |
| High resolution limit [Å] | 2.510 | 2.510 |
| Number of reflections | 19458 | 1920 |
| <I/σ(I)> | 15.35 | |
| Completeness [%] | 98.0 | 94.32 |
| Redundancy | 5.5 | 5.2 |
| CC(1/2) | 0.822 | 0.222 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298.15 | 8% v/v Tacsimate (pH 7.0) and 20 % w/v Polyethylene glycol 3,350 |
