7MY4

Crystal Structure of the SPA17 Docking and Dimerization Domain from Danio rerio

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2020-02-13
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	P 32
Unit cell lengths	60.957, 60.957, 89.021
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.030 - 1.720
R-factor	0.1551
Rwork	0.154
R-free	0.16450
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2izx
RMSD bond length	0.008
RMSD bond angle	0.923
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.030	1.770
High resolution limit [Å]	1.720	1.720
Rmerge		0.269
Rmeas	0.098
Rpim	0.035
Number of reflections	38456	2519
<I/σ(I)>	1.02
Completeness [%]	98.5	95
Redundancy	7.6	5.6
CC(1/2)	0.993

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	0.1 M HEPES pH 7.5, 0.5 M magnesium formate dihydrate