7MXU
Crystal structure of human exonuclease 1 Exo1 (WT) in complex with 5' flap DNA (cf2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 12.3.1 |
| Synchrotron site | ALS |
| Beamline | 12.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-10-23 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 73.169, 73.169, 182.621 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.733 - 3.042 |
| R-factor | 0.233 |
| Rwork | 0.230 |
| R-free | 0.26870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5v05 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.847 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.100 |
| High resolution limit [Å] | 3.042 | 3.050 |
| Rmerge | 0.063 | 0.646 |
| Number of reflections | 9925 | 470 |
| <I/σ(I)> | 29.46 | |
| Completeness [%] | 97.4 | |
| Redundancy | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 290 | 100 mM sodium acetate, pH 7.0; 10 mM KCl; 2-4% PEG 4000 |
