7MXQ

Crystal structure of human exonuclease 1 Exo1 (WT) in complex with 5' recessed-end DNA (r-1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 12.3.1
Synchrotron site	ALS
Beamline	12.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-10-02
Detector	ADSC QUANTUM 315r
Wavelength(s)	1
Spacegroup name	P 43 21 2
Unit cell lengths	74.058, 74.058, 180.807
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.583 - 3.230
R-factor	0.2073
Rwork	0.203
R-free	0.24890
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5v05
RMSD bond length	0.002
RMSD bond angle	0.392
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.290
High resolution limit [Å]	3.230	3.230
Rmerge	0.096	0.678
Number of reflections	7864	405
<I/σ(I)>	21.75
Completeness [%]	90.5
Redundancy	5.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		290	100 mM sodium acetate, pH 7.0; 10 mM KCl; 2-4% PEG 4000