7MXK

Crystal structure of the S/T protein kinase PknG from Corynebacterium glutamicum (residues 130-433) in complex with AMP-PNP, isoform 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-02-22
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.976251
Spacegroup name	P 21 21 21
Unit cell lengths	37.807, 54.594, 146.492
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.770 - 1.990
R-factor	0.2102
Rwork	0.209
R-free	0.23700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7mxb
RMSD bond length	0.003
RMSD bond angle	0.613
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.830	2.040
High resolution limit [Å]	1.990	1.990
Rmerge	0.067	0.654
Rmeas	0.073	0.725
Rpim	0.029	0.306
Number of reflections	21235	1207
<I/σ(I)>	17.1
Completeness [%]	98.4	84.9
Redundancy	6	5.2
CC(1/2)	0.999	0.751

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		291	100 mM Tris-HCl, 27-30% w/v PEG 4 k, 200 mM MgCl2, pH 8.8