7MX9

Crystal structure of the SARS-CoV-2 ORF8 accessory protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	93.15
Detector technology	IMAGE PLATE
Collection date	2021-02-02
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.541870
Spacegroup name	P 42 21 2
Unit cell lengths	51.349, 51.349, 74.093
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.040 - 2.600
R-factor	0.2487
Rwork	0.243
R-free	0.29660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7jtl
RMSD bond length	0.004
RMSD bond angle	0.748
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.18.2-3874_3874: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.200	2.720
High resolution limit [Å]	2.600	2.600
Rmerge	0.166	0.796
Number of reflections	3379	400
<I/σ(I)>	11.8
Completeness [%]	100.0
Redundancy	13
CC(1/2)	0.997	0.932

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.8	293.15	0.2 M Lithium chloride, 20% (w/v) Polyethylene glycol 3350