7MWX
Structure of the core ectodomain of the hepatitis C virus envelope glycoprotein 2 with tamarin CD81
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-12-07 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 76.954, 127.774, 212.374 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 53.093 - 3.320 |
| R-factor | 0.2414 |
| Rwork | 0.239 |
| R-free | 0.28310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4web |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.668 |
| Data reduction software | DIALS |
| Data scaling software | DIALS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.740 | 3.500 |
| High resolution limit [Å] | 3.320 | 3.320 |
| Number of reflections | 31609 | 4559 |
| <I/σ(I)> | 6.8 | |
| Completeness [%] | 99.5 | |
| Redundancy | 13.2 | |
| CC(1/2) | 0.990 | 0.390 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 277 | 0.2M sodium acetate pH 4.5, 4% v/v tacsimate pH 4.0, 12% v/v PEG Smear Medium |
