7MWA
Crystal structure of 2-octaprenyl-6-methoxyphenol hydroxylase UbiH from Acinetobacter baumannii, apoenzyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2019-07-24 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.548 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 199.378, 71.680, 111.261 |
| Unit cell angles | 90.00, 118.94, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.600 |
| R-factor | 0.2021 |
| Rwork | 0.200 |
| R-free | 0.24600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4k22 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.492 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MrBUMP |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.640 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.086 | 0.506 |
| Rpim | 0.047 | 0.318 |
| Number of reflections | 42324 | 1940 |
| <I/σ(I)> | 12.33 | 1.12 |
| Completeness [%] | 99.0 | 89.8 |
| Redundancy | 4.1 | 3 |
| CC(1/2) | 0.838 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 298 | 0.2 M magnesium chloride, 0.1 M sodium citrate pH 5, 10% (w/v) PEG 20K. Cryoprotectant 25% (w/v) ethylene glycol |
