7MW0

Crystal structure of Homo sapiens NUP93 solenoid (residues 174-819)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-18
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	P 1 21 1
Unit cell lengths	74.870, 66.070, 79.180
Unit cell angles	90.00, 116.28, 90.00

Refinement procedure

Resolution	40.000 - 2.000
R-factor	0.2262
Rwork	0.225
R-free	0.25440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5hb3
RMSD bond length	0.002
RMSD bond angle	0.452
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.071
High resolution limit [Å]	2.000	2.000
Rmerge	0.088	2.537
Rmeas	0.090	2.598
Rpim	0.020	0.555
Number of reflections	46714	4598
<I/σ(I)>	26	1.54
Completeness [%]	99.1	98.27
Redundancy	20.9	21.5
CC(1/2)	1.000	0.733

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	294	0.075 M TRIS pH 8.5, 19 % (w/v) PEG 20000