7MVT

Crystal structure of the Chaetomium thermophilum Nup192-Nic96 complex (Nup192 residues 185-1756; Nic96 residues 187-301)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-01-08
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.14012
Spacegroup name	P 41 21 2
Unit cell lengths	76.776, 76.776, 712.505
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.570 - 3.600
R-factor	0.261
Rwork	0.258
R-free	0.29670
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5hb4
RMSD bond length	0.002
RMSD bond angle	0.443
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	3.730
High resolution limit [Å]	3.600	3.600
Rmerge	0.142	1.832
Rmeas	0.146	1.882
Rpim	0.032	0.416
Number of reflections	46821	2539
<I/σ(I)>	12.4	1.66
Completeness [%]	99.6	97.74
Redundancy	20.8	18.9
CC(1/2)	1.000	0.778

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	294	0.05 M HEPES pH 7.5, 0.1 M ammonium sulfate, 8 % (w/v) PEG 3350, 1 % (v/v) 2-propanol