7MU4

Crystal Structure of HPV L1-directed D24.M01Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-28
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	52.021, 77.743, 105.029
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.620 - 1.830
R-factor	0.1757
Rwork	0.174
R-free	0.21140
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6apb
RMSD bond length	0.011
RMSD bond angle	1.230
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.630	1.900
High resolution limit [Å]	1.830	1.830
Number of reflections	33966	1715
<I/σ(I)>	19.9
Completeness [%]	89.0
Redundancy	5.8
CC(1/2)	0.990	0.714

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	295	0.1 M HEPES pH 7.5, 10% w/v Polyethylene glycol 6000, 5% v/v (+/-)-2-Methyl-2,4-pentanediol