7MSN

SunS glycosin S-glycosyltransferase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2011-04-10
Detector	MAR scanner 300 mm plate
Wavelength(s)	0.9787
Spacegroup name	P 43 21 2
Unit cell lengths	108.730, 108.730, 210.697
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.380 - 3.000
R-factor	0.2079
Rwork	0.204
R-free	0.27960
Structure solution method	SAD
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.100
High resolution limit [Å]	3.000	3.000
Number of reflections	47618	2476
<I/σ(I)>	20.1
Completeness [%]	91.2
Redundancy	7.4
CC(1/2)	0.998	0.800

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		283	PEG 4000 50 mM HEPES, pH=7.5