7MSL

Structure of anti-CRISPR AcrIIC4 from Haemophilus parainfluenzae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	Cu FINE FOCUS
Temperature [K]	100
Detector technology	CCD
Collection date	2018-02-02
Detector	RIGAKU
Wavelength(s)	1.54
Spacegroup name	P 4 21 2
Unit cell lengths	82.450, 82.450, 27.340
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.870 - 1.730
R-factor	0.2422
Rwork	0.239
R-free	0.27360
Structure solution method	SAD
RMSD bond length	0.004
RMSD bond angle	0.536
Data reduction software	MOSFLM
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.870	1.800
High resolution limit [Å]	1.730	1.730
Number of reflections	10268	990
<I/σ(I)>	17.32
Completeness [%]	99.9
Redundancy	2
CC(1/2)	0.998	0.670

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.2	294.15	35% PEG 600, 0.1M NaHPO4/citric acid pH 4.2