7MRO

Zebrafish CNTN4 FN1-FN3 domains

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-14
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	44.700, 56.880, 130.940
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.920 - 1.870
R-factor	0.1772
Rwork	0.174
R-free	0.21590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5e4s
RMSD bond length	0.006
RMSD bond angle	0.843
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	56.880	1.910
High resolution limit [Å]	1.870	1.870
Rmerge	0.131	0.275
Rmeas	0.136	0.285
Rpim	0.037	0.075
Number of reflections	28351	1791
<I/σ(I)>	12.7	8.5
Completeness [%]	100.0	100
Redundancy	13.2	13.9
CC(1/2)	0.994	0.978

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	100mM Na-acetate pH 5.5, 30% (w/v) PEG 8,000