7MRM

Chicken CNTN3 APP complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-02-25
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	69.669, 94.500, 174.149
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.870 - 2.750
R-factor	0.1958
Rwork	0.193
R-free	0.23130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ktm 5e4q
RMSD bond length	0.003
RMSD bond angle	0.496
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.870	2.900
High resolution limit [Å]	2.750	2.750
Rmerge	0.179	0.699
Rmeas	0.194	0.753
Rpim	0.073	0.275
Number of reflections	30550	4407
<I/σ(I)>	7.8	2.9
Completeness [%]	99.9	100
Redundancy	6.9	7.3
CC(1/2)	0.987	0.891

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	100 mM Na-acetate pH 5.5, 1.1 M Li2SO4, 3% (w/v) sucrose