7MRK

Chicken CNTN4 APP complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2017-11-10
Detector	RAYONIX MX300-HS
Wavelength(s)	1.0
Spacegroup name	I 1 2 1
Unit cell lengths	128.450, 65.510, 158.294
Unit cell angles	90.00, 92.92, 90.00

Refinement procedure

Resolution	32.410 - 2.050
R-factor	0.1844
Rwork	0.183
R-free	0.22480
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ktm 5e4s
RMSD bond length	0.003
RMSD bond angle	0.591
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	64.140	2.090
High resolution limit [Å]	2.050	2.050
Rmerge	0.101	0.772
Rmeas	0.111	0.839
Rpim	0.044	0.326
Number of reflections	82428	4511
<I/σ(I)>	10	2.3
Completeness [%]	99.7	100
Redundancy	6.4	6.5
CC(1/2)	0.995	0.805

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	150 mM (NH4)2SO4, 50 mM Na-cacodylate pH 6.5, 30% (v/v) PEG 550 MME