7MRJ

Crystal structure of a novel ubiquitin-like TINCR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-29
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979
Spacegroup name	P 43 21 2
Unit cell lengths	67.676, 67.676, 89.528
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	53.990 - 2.120
R-factor	0.1928
Rwork	0.188
R-free	0.23620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4hwi
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	53.990	2.210
High resolution limit [Å]	2.120	2.120
Rmerge	0.140	0.660
Number of reflections	12340	1186
<I/σ(I)>	15
Completeness [%]	100.0	99.9
Redundancy	24.4
CC(1/2)	0.980	0.830

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	5	277	0.1 M Magnesium sulfate heptahydrate, 0.1 M Sodium Acetate, and 20% (w/v) PEG 1000