7MPX

CmcC E36G mutant from Type II Cut MCP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-08-18
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9786
Spacegroup name	P 1 21 1
Unit cell lengths	66.570, 61.000, 66.610
Unit cell angles	90.00, 119.92, 90.00

Refinement procedure

Resolution	57.730 - 2.100
R-factor	0.2419
Rwork	0.237
R-free	0.28320
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	D_1000255588
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.18.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	57.730	57.730	2.150
High resolution limit [Å]	2.100	9.370	2.100
Rmerge	0.105	0.042	1.430
Rmeas	0.118	0.047	1.597
Total number of observations	134627
Number of reflections	26358	325	1920
<I/σ(I)>	6.83	23.29	0.87
Completeness [%]	96.1	97	95.3
Redundancy	5.108	4.822	4.968
CC(1/2)	0.998	0.999	0.730

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		294	0.1 M SPG buffer pH 6.0, 25% PEG 1500