7MP9

Crystal structure of the cytosolic domain of Tribolium castaneum PINK1 phosphorylated at Ser205 in complex with ADP analog

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-03-14
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97949
Spacegroup name	P 61 2 2
Unit cell lengths	52.736, 52.736, 547.944
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.670 - 2.800
R-factor	0.2497
Rwork	0.247
R-free	0.30650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5yj9
RMSD bond length	0.003
RMSD bond angle	0.548
Data reduction software	autoPROC
Data scaling software	STARANISO
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.670	2.920
High resolution limit [Å]	2.800	2.800
Number of reflections	9568	478
<I/σ(I)>	10.6	2.3
Completeness [%]	93.4	100
Redundancy	34.9
CC(1/2)	0.996	0.841

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		288	80mM MES (pH 6.5), 0.16M (NH4)2 SO4, 16% PEG 8K.