7MNZ

Crystal Structure of Nup358/RanBP2 Ran-binding domain 4 in complex with Ran-GPPNHP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-03-02
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.03317
Spacegroup name	P 31
Unit cell lengths	142.690, 142.690, 96.650
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.360 - 2.350
R-factor	0.1854
Rwork	0.183
R-free	0.22520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1rrp
Data reduction software	XDS
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX (1.19.1)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.360	29.360	2.430
High resolution limit [Å]	2.350	5.060	2.350
Rmeas	0.154	0.061	1.835
Rpim	0.048	0.019	0.591
Total number of observations	935004	90644	86810
Number of reflections	91578	9162	9170
<I/σ(I)>	12.4	30.3	1.8
Completeness [%]	99.9	99.9	99.8
Redundancy	10.2	9.9	9.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	294	20% w/v PEG3350, 0.1 M ammonium sulfate, 0.1 M HEPES