7MNY

Crystal Structure of Nup358/RanBP2 Ran-binding domain 3 in complex with Ran-GPPNHP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-18
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00000
Spacegroup name	P 21 21 21
Unit cell lengths	110.200, 136.031, 158.755
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.930 - 2.700
R-factor	0.2167
Rwork	0.214
R-free	0.26180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1rrp
Data reduction software	DIALS
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX (1.18.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.930	29.940	2.800
High resolution limit [Å]	2.700	5.800	2.700
Rmeas	0.234	0.080	1.881
Rpim	0.063	0.022	0.507
Total number of observations	900779	90868	88275
Number of reflections	66178	6945	6497
<I/σ(I)>	8.4	18.9	1.5
Completeness [%]	100.0	100	99.8
Redundancy	13.6	13.1	13.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		294	20% w/v PEG3350, 0.2 M sodium formate