7MNX

Crystal Structure of Nup358/RanBP2 Ran-binding domain 2 in complex with Ran-GPPNHP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-25
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	P 21 21 21
Unit cell lengths	94.173, 133.975, 182.516
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.820 - 2.400
R-factor	0.1928
Rwork	0.192
R-free	0.23010
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1rrp
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.18.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.820	29.830	2.490
High resolution limit [Å]	2.400	5.160	2.400
Rmeas	0.174	0.077	2.504
Rpim	0.047	0.021	0.705
Total number of observations	1189731	124166	107541
Number of reflections	89794	9381	8716
<I/σ(I)>	9.7	23.3	1.1
Completeness [%]	98.8	98.9	96.9
Redundancy	13.2	13.2	12.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		294	15% w/v PEG3350, 0.125 M magnesium formate