7MNT

Crystal Structure of the ZnF5 or ZnF6 of Nucleoporin NUP358/RanBP2 in complex with Ran-GDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-11-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.2828
Spacegroup name	P 21 21 21
Unit cell lengths	53.020, 94.270, 108.160
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.520 - 2.450
R-factor	0.2145
Rwork	0.212
R-free	0.25660
Structure solution method	SAD
Data reduction software	XDS
Data scaling software	xia2
Phasing software	AutoSol
Refinement software	PHENIX (1.18.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.520	29.520	2.540
High resolution limit [Å]	2.450	5.270	2.450
Rmeas	0.119	0.057	3.284
Rpim	0.023	0.012	0.637
Total number of observations	524547	49374	52237
Number of reflections	20617	2211	2020
<I/σ(I)>	21.8	54.5	1.6
Completeness [%]	100.0	100	100
Redundancy	25.4	22.3	25.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	294	19 % (w/v) PEG 3,350; 0.1M Bis-Tris