7MNP

Crystal Structure of the ZnF2 of Nucleoporin NUP358/RanBP2 in complex with Ran-GDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-10-29
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0332
Spacegroup name	P 21 21 21
Unit cell lengths	60.230, 79.840, 108.750
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.020 - 2.050
R-factor	0.196
Rwork	0.194
R-free	0.23140
Structure solution method	SAD
Data reduction software	XDS
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX (1.18.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.020	29.030	2.130
High resolution limit [Å]	2.050	4.420	2.050
Rmeas	0.127	0.045	1.468
Rpim	0.038	0.013	0.467
Total number of observations		36729	28669
Number of reflections	62430	3537	3140
<I/σ(I)>	12.5	35.9	1.6
Completeness [%]	99.4	99.7	95.3
Redundancy	10.9	10.4	9.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.4	294	17 % (w/v) PEG 3,350; 0.1M Bis-Tris