7MMT
Crystal Structure of the Class Ie Ribonucleotide Reductase Beta Subunit from Aerococcus urinae with Cu(I) bound (Cu chloride soak)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-03-08 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.614, 46.721, 133.786 |
| Unit cell angles | 90.00, 90.49, 90.00 |
Refinement procedure
| Resolution | 38.330 - 1.540 |
| R-factor | 0.1848 |
| Rwork | 0.184 |
| R-free | 0.20680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6ebo |
| RMSD bond length | 0.003 |
| RMSD bond angle | 1.145 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.570 |
| High resolution limit [Å] | 1.540 | 4.180 | 1.540 |
| Rmerge | 0.071 | 0.045 | 0.589 |
| Rmeas | 0.077 | 0.049 | 0.649 |
| Rpim | 0.030 | 0.019 | 0.268 |
| Total number of observations | 624403 | ||
| Number of reflections | 96895 | 5110 | 4553 |
| <I/σ(I)> | 8.4 | ||
| Completeness [%] | 98.4 | 99.8 | 93.6 |
| Redundancy | 6.4 | 6.2 | 5.1 |
| CC(1/2) | 0.996 | 0.871 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 18% (w/v) PEG 3350, 0.2 M magnesium chloride |
