7MMN

Crystal Structure of the Prefusion RSV F Glycoprotein bound by human antibody AM14

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-13
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1
Spacegroup name	C 1 2 1
Unit cell lengths	223.423, 183.002, 135.785
Unit cell angles	90.00, 103.14, 90.00

Refinement procedure

Resolution	42.330 - 3.570
R-factor	0.2035
Rwork	0.202
R-free	0.24530
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4mmu 4zyk
RMSD bond length	0.003
RMSD bond angle	0.644
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.330	3.700
High resolution limit [Å]	3.570	3.570
Rmerge	0.200	0.890
Rmeas	0.250
Rpim	0.130
Number of reflections	62699	2848
<I/σ(I)>	8.3	1.74
Completeness [%]	88.2
Redundancy	3.6
CC(1/2)	0.960	0.510

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	0.2M NaCl, 0.1M MES pH 6.0, 20% w/v PEG 2000 MME