7MLW
Burkholderia sp. TJI49 Guanidine-I riboswitch
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-04-14 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9919 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 51.703, 51.703, 266.728 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 6.990 - 2.700 |
| R-factor | 0.2232 |
| Rwork | 0.218 |
| R-free | 0.27030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5t83 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.155 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19_4080) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 7.000 | 2.850 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.070 | 0.730 |
| Number of reflections | 11350 | 936 |
| <I/σ(I)> | 25.7 | 4.1 |
| Completeness [%] | 98.0 | 99.6 |
| Redundancy | 18.2 | 18.2 |
| CC(1/2) | 0.990 | 0.920 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 288 | 50 mM HEPES (pH 7.5), 200 mM ammonium sulfate, 10 mM strontium chloride, 30% PEG 3350, 3% isopropanol |
