7MLJ
Crystal structure of Thermus thermophilus reiterative transcription complex with 4nt oligo-G RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-05-31 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97946 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 186.309, 104.656, 298.413 |
| Unit cell angles | 90.00, 98.12, 90.00 |
Refinement procedure
| Resolution | 46.210 - 3.750 |
| R-factor | 0.266 |
| Rwork | 0.265 |
| R-free | 0.28330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4g7h |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.515 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.810 |
| High resolution limit [Å] | 3.750 | 3.750 |
| Rmerge | 0.103 | 1.031 |
| Number of reflections | 57607 | 2910 |
| <I/σ(I)> | 14.522 | |
| Completeness [%] | 97.9 | 99 |
| Redundancy | 4.2 | 4.2 |
| CC(1/2) | 0.837 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.4 | 295 | 0.1 M Tris-HCl pH8.4, 180 mM potassium chloride, 50 mM Magnesium chloride, 9% (m/v) PEG 4000 |
