7MK5
Crystal structure of Escherichia coli ClpP covalently inhibited by clipibicyclene
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08B1-1 |
| Synchrotron site | CLSI |
| Beamline | 08B1-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2021-02-05 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.97895 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 94.331, 190.230, 169.884 |
| Unit cell angles | 90.00, 93.61, 90.00 |
Refinement procedure
| Resolution | 45.410 - 2.950 |
| R-factor | 0.2124 |
| Rwork | 0.211 |
| R-free | 0.24790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | ITYF |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.267 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.410 | 3.055 |
| High resolution limit [Å] | 2.950 | 2.950 |
| Rmerge | 0.175 | 1.250 |
| Rpim | 0.102 | 0.720 |
| Number of reflections | 125212 | 12429 |
| <I/σ(I)> | 6.9 | 1.2 |
| Completeness [%] | 99.7 | 99.7 |
| Redundancy | 3.9 | |
| CC(1/2) | 0.988 | 0.423 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 0.1 M sodium acetate, 30% MPD |
