7MK1

Structure of a protein-modified aptamer complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-07-07
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	77.126, 86.632, 87.126
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	57.750 - 1.900
R-factor	0.1901
Rwork	0.188
R-free	0.21960
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3og8
RMSD bond length	0.019
RMSD bond angle	4.332
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	57.750	1.968
High resolution limit [Å]	1.900	1.900
Rmerge	0.030
Rmeas	0.043
Rpim	0.030
Number of reflections	46578	4608
<I/σ(I)>	10.72
Completeness [%]	99.8	99.96
Redundancy	2	2
CC(1/2)	0.999	0.549

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		285.15	30% PEG3350, 0.01 M Na acetate pH 4.8