7MK0
Trypanosoma cruzi Nucleoside Diphosphate Kinase 1 form a quinary multihexameric structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE D03B-MX1 |
| Synchrotron site | LNLS |
| Beamline | D03B-MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-09-30 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.45 |
| Spacegroup name | P 3 |
| Unit cell lengths | 127.842, 127.842, 275.494 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 - 3.500 |
| R-factor | 0.2691 |
| Rwork | 0.269 |
| R-free | 0.27770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bbc |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (5.6.0117) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 86.380 | 3.690 |
| High resolution limit [Å] | 3.000 | 3.498 |
| Rmerge | 0.340 | 1.121 |
| Number of reflections | 58612 | 9287 |
| <I/σ(I)> | 2.7 | |
| Completeness [%] | 92.3 | 99.9 |
| Redundancy | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.3 | 277 | PEG3350 20% Magnesium chloride 200mM |
