7MJY

MiaB in the complex with s-adenosyl-L-homocysteine and RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-07-24
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0033
Spacegroup name	P 21 21 21
Unit cell lengths	53.834, 79.982, 110.137
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.370 - 1.860
R-factor	0.1758
Rwork	0.174
R-free	0.20880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2qgq
RMSD bond length	0.006
RMSD bond angle	1.292
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.890
High resolution limit [Å]	1.860	5.050	1.860
Rmerge	0.152	0.064	0.918
Rmeas	0.163	0.069	1.014
Rpim	0.058	0.024	0.421
Total number of observations	303282
Number of reflections	40640	2224	1971
<I/σ(I)>	4.8
Completeness [%]	100.0	100	99.6
Redundancy	7.5	7.5	5.1
CC(1/2)		0.997	0.681

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	100 mM sodium cacodylate, pH 5.5, 13% PEG 4000, and 3% 1,6-hexanediol