7MFB
Crystal structure of antibody 10E8v4 Fab - light chain H31F variant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 93 |
Detector technology | PIXEL |
Collection date | 2020-03-09 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.0000 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 141.800, 56.231, 69.261 |
Unit cell angles | 90.00, 100.85, 90.00 |
Refinement procedure
Resolution | 40.040 - 1.740 |
R-factor | 0.2237 |
Rwork | 0.223 |
R-free | 0.24590 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5iq9 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.315 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.780 |
High resolution limit [Å] | 1.740 | 4.750 | 1.750 |
Rmerge | 0.100 | 0.042 | 0.596 |
Rmeas | 0.118 | 0.050 | 0.680 |
Rpim | 0.061 | 0.027 | 0.316 |
Number of reflections | 48111 | 2173 | 2394 |
<I/σ(I)> | 5.6 | ||
Completeness [%] | 88.2 | 76.7 | 87.3 |
Redundancy | 3.3 | 3 | 3.4 |
CC(1/2) | 0.994 | 0.826 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 12% PEG3350, 20% iso-propanol, 0.1M Tris-HCl 8.5 |