7MCY

Crystal structure of Staphylococcus aureus Cystathionine gamma lyase, Holoenzyme with bound NL3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-05-10
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	0.92013
Spacegroup name	I 41 2 2
Unit cell lengths	105.448, 105.448, 287.772
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.710 - 1.720
R-factor	0.1584
Rwork	0.158
R-free	0.16410
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ixz
RMSD bond length	0.007
RMSD bond angle	0.992
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.750
High resolution limit [Å]	1.720	1.720
Rmerge	0.117	0.846
Rpim	0.031	0.288
Number of reflections	85730	3829
<I/σ(I)>	20.3	1.4
Completeness [%]	99.4	89.8
Redundancy	14.4	6.3
CC(1/2)	0.998	0.826

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291	0.1 M Tris-HCl, pH 7.5, 1.4 M Tri Sodium Citrate