7MCR
Human Apex/Ref1 homodimer formed under oxidative condition
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER TURBO X-RAY SOURCE |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-07-26 |
| Detector | Bruker PHOTON II |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 77.042, 44.794, 77.436 |
| Unit cell angles | 90.00, 92.27, 90.00 |
Refinement procedure
| Resolution | 27.843 - 1.900 |
| R-factor | 0.1699 |
| Rwork | 0.163 |
| R-free | 0.23260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5cfg |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.871 |
| Data reduction software | XPREP |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.843 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.674 | |
| Rmeas | 0.248 | 0.844 |
| Rpim | 0.132 | 0.503 |
| Number of reflections | 21090 | 2010 |
| <I/σ(I)> | 21.2 | 2.8 |
| Completeness [%] | 99.2 | 94.9 |
| Redundancy | 3.3 | 2.5 |
| CC(1/2) | 0.943 | 0.690 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293.15 | 100 mM MES/ sodium hydroxide, 200 mM calcium acetate, 20% W/V PEG 8000 |
